Rapid Genome Sequencing (RPDGENTRIO)

CPT Code(s): 81425

Service Code (IU Health): 53124822, 53124830

Ordering Recommendation: Genome testing for critically ill patients

Synonyms: rapid genome, genome test, whole genome sequencing

Methodology: Next generation sequencing (NGS)

Performed: Weekly

Reported: 5-14 days from the time the laboratory receives the specimens

For test ordering, please contact the laboratory at 317-278-0100.

Specimen Type and Handling

Type preferred	Blood Cord blood Saliva Buccal swab Genomic DNA (isolated in a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by the CAP and/or the CMS)
Other acceptable but not recommended types	N/A
Collection container	Oragene collection kit (saliva) Oragene collection kit (buccal swab) EDTA (purple top) capped tube (blood)
Optimum volume of samples	Saliva: Oragene DNA self-collection kit. Collect to the line (≥2 ml) Buccal: ORAcollect*Dx (OCD-100) Blood: 3-5 mL (2 years to adult) 2-3 mL (newborn to 2 years) Cord blood: 1-2 mL Genomic DNA: 10-15 μg
Minimum volume of samples	DNA: minimum concentration of 50ng/μl; A260/A280 of ~1.7
Unacceptable specimens and actions to take	Grossly hemolyzed or clotted specimens are not accepted. Blood should not be sent from patients who have had a bone marrow transplant or recent blood transfusion.
Compromising physical characteristics	None known