10x Genomics Single Cell Assays

Assay	Universal 3' Gene Expression	Universal 5' Gene Expression	Flex Gene Expression	Epi ATAC	Epi Multiome ATAC + Gene Expression
Overview	Whole transcriptome coverage	Whole transcriptome coverage	Protein coding gene coverage	Chromatin accessibility	Simultaneous messurement of gene expression and chromatin accessibility from the same nucleus
Chemistry	Reverse transcription-based	Reverse transcription-based	Probe-based
Compatible species	Agnostic	Agnostic	Human, mouse, other species with custom probes	Human, mouse, rat, non-human primate, marine, plant, insect	Human, mouse, rat, non-human primate, marine, plant, insect
Compatible sample types	Cell suspensions Nuclei suspensions Flow-sorted cells Fixed human PBMCs Fresh tissue Frozen tissue Organoids	Cell suspensions Nuclei suspensions Flow-sorted cells Fixed human PBMCs Fresh tissue Frozen tissue Organoids	Cell suspensions Nuclei suspensions Flow-sorted cells Fixed whole blood Fresh tissue Frozen tissue FFPE tissue PFA-fixed tissue Organoids	Cell suspensions Nuclei suspensions Fresh tissue Frozen tissue Organoids	Cell suspensions Nuclei suspensions Fresh tissue Frozen tissue Organoids
Multiomic capabilities	3' gene expression Cell surface protein	5' geen expression TCR & BCR sequencing Cell surface protein CRISPR screening Antigen specificity	Probe-based gene expression Cell surface protein Intracellular protein CRISPR screening	Chromatin accessibility	3' gene expression Chromatin accessibility
Throughput	Singleplex 20K cells/sample 160K cells/chip On-chip multiplexing 5K cells/sample 40K cells/chip Up to 4 samples/channel Output: 8 samples/chip Hashing/Feature barcoding 8 channels/chip 60K cells/channel 480K cells/chip	Singleplex 20K cells/sample 160K cells/chip On-chip multiplexing 5K cells/sample 40K cells/chip Up to 4 samples/channel Output: 8 samples/chip Hashing/Feature barcoding 8 channels/chip 60K cells/channel 480K cells/chip	Singleplex 20K cells/sample 160K cells/chip In-line multiplex 20K cells/sample Up to 16 samples/channel 2.56M cells/chip	Singleplex 10K nuclei/sample	Singleplex 10K nuclei/sample